Programme April 2018

The Copenhagen Workshop on Cryopreservation of Ovarian Tissue - April 24-26, 2018.

Tuesday 24th

08.30 – 09.00 Breakfast

09:00 – 09:15 Welcome and introduction /Claus Yding Andersen

09:15 – 10:15 Cryopreservation of human ovarian tissue (Part I): Technique & protocols /Claus Yding Andersen

10.15 – 10:45 Who are eligible for fertility preservation? /Anne-Mette Bay Bjørn

10:45 – 11:00 Coffee break and fruit

11:00 – 11:30 Cryopreservation of ovarian tissue in children /Anne-Mette Bay Bjørn

11:30 – 11:50 Cryopreservation of testicular tissue for fertility preservation /Lihua Dong

11:50 – 12:00 Overview of the afternoon sessions and a short introduction to hands-on sessions with bovine ovaries  /Stine Gry Kristensen 

12:00 – 13:00 Lunch

13:00 – 15:30 In the lab: Hands-on sessions; preparing bovine ovarian cortex for cryopreservation and freezing the ovarian tissue by controlled slow-freezing

15:30 – 16:00 Coffee break and cake

16:00 – 17:00 Cryopreservation of human ovarian tissue (Part 2): Technique & protocols /Claus Yding Andersen

17:00 – 17:30 In the lab: Cryo-tubes are sealed and placed into liquid nitrogen tanks

Wednesday 25th

09:00 – 10:15 Fundamentals of cryobiology and vitrification versus slow-freezing /Claus Yding Andersen

10:15 – 10:45 Validation of the cryopreservation protocol /Stine Gry Kristensen

10:45 – 11:00 Coffee break and fruit

11:00 – 11:30 Collection and transplantation of ovarian tissue – the surgical perspective /Erik Ernst 

11:30 – 11:50 Activation of resting ovarian follicles - a novel approach for fertility treatment and preservation /Susanne Pors 

11:50 – 12:10 Using ovarian tissue for postponing menopause /Stine Gry Kristensen

12:10 – 13:10 Lunch

13:10 – 15:00 In the lab: Hands-on session; preparing bovine ovarian tissue for cryopreservation 

15:00 – 15:15 Coffee break and cake

15:15 – 15:45 The Danish cohort and transportation of the ovarian tissue prior to freezing /Claus Yding Andersen

15:45 – 16:15 IVF-outcomes: Managing and stimulating patients transplanted with ovarian tissue /Peter Humaidan

18:00 - Dinner and Drinks

Thursday 26th

09:00 – 10:45 In the lab: Hands-on session; thawing cryopreserved tissue and viability assessment 

10:45 – 11:00 Coffee break and fruit

11.00 – 11.30 Risk of malignant cell contamination /Claus Yding Andersen

11.30 – 12:00 Isolation and culture of pre-antral follicles from human ovarian medulla tissue /Stine Gry Kristensen

12:00 – 13:00 Lunch

13:00 – 13:30 In the lab: Final viability assessment of the cryopreserved/thawed bovine ovarian tissue 

13:30 – 14:30 Round up and evaluation

End of workshop

Lecturers

Claus Yding Andersen, Professor
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
claus.yding.andersen@regionh.dk

Anne-Mette Bay Bjørn, MD, PhD
Afdeling for Kvindesygdomme og Fødsler, Aarhus Universitetshospital, Skejby, Denmark
annbjoer@rm.dk

Erik Ernst, Professor
Department of Gynaecology and Obstetrics, Aarhus University Hospital, Skejby, Denmark
erik.ernst@skejby.rm.dk

Peter Humaidan, Professor
Fertility Clinic, Skive Regional Hospital and Faculty of Health, Aarhus University, Denmark
peter.humaidan@midt.rm.dk

Stine Gry Kristensen, Post Doc
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
stine.gry.kristensen@regionh.dk

Susanne Pors, Post Doc
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
susanne.elisabeth.pors@regionh.dk

Lihua Dong, Post Doc
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
lihua.dong@regionh.dk


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